Yeast of the genus Malassezia belong to the normal microflora of human. However these yeasts are associated with various skin disorders including seborrheic dermatitis, malassezia versicolor, certain form of folliculitis, and even atopic dermatitis. We have identified genetic difference between 12 Malassezia species (Malassezia globosa, M. restricta, M. obtuse, M. sloofiae, M. furfur, M. sympodialis, M. dermatis, M. pachydermatis, M. equina, M. nana, M. japonica, M. yamatoensis). DNA characterization of twelve Malassezia species CBS type strain was performed by using D1/D2 26S gene sequencing analysis to understand their phylogenetic relationships and to analyze their specific genetic variation. Also, We isolated the azole-resistant Malassezia species from Patients with Recalcitrant Seborrheic Dermatitis and searched mutation site of azole-resistant Malassezia species and type strain was compared. Minimal inhibitory concentration (MIC) was measured in both standard strain and patient originated resistant strain. Resistant strain presented higher MIC level than standard strain. And these two strains did not show any difference in Full Spectrum Light(FSL) irradiation (BMC, Korea) dose. Therefore, FSL treatment might be an treatment modality for classical seborrheic dermatitis and resistant seborrheic dermatitis. This study is supported by a grant of the Korea Healthcare technology R&D project, Ministry of Health & welfare (A080065).