Objective: To construct RNA interference (RNAi) expression vector targeting to androgen receptor (AR) gene and investigate whether the recombined plasmids could regulate the expression of AR in human dermal papilla cell (DPC).
Methods: Three AR-specific siRNAs were selected and RNAi expression vectors were constructed, then the DPCs were transfected with the recombined plasmids by lipofectin. AR protein expression was detected by western blot. Then the proliferation of DPC was detected by MTT and 3H-TdR incorporation and the apoptosis of DPC was also detected by flow cytometer.
Results: 1The eukaryotic expression vector Ri-AR were constructed and was transfected into the DPCs successfully. 2The results of western blot showed that AR RNAi decreased the level of AR expression in DPCs (P<0.05). 3The results of MTT assay and 3H-TdR incorporation suggested that blocking the AR with the RNAi expression vector could not decrease the proliferation of DPC efficiently and with no affect on the apoptosis of DPC.
Conclusion: The results suggested that RNA interference could inhibit AR expression in DPCs with no effects on the proliferation and the apoptosis of DPC. It might be significant for the androgenic alopecia gene therapy.