Copper-conjugated synthetic tripeptide (Alotide) stimulating the growth of hair dermal papilla cell

  • Dr Seong Kim, Department of Dermatology, Chonnam National University Medical School & Neurocosmetic, inc., Gwangju, Korea
  • Dr Min-Gyu Soung, Peptron, Inc. Daejeon, Korea
  • Dr Keedon Park, Peptron, Inc., Daejeon, Korea
  • Dr Jong-Sung Park, Department of Physiology, Chonnam National University Medical School, Gwangju, Korea
  • Chaejin Lim, Peptron, Inc., Korea

Many therapeutic modalities has been developed to improve and enhance the hair growth. Among many chemical and natural compounds, some potentials were observed from certain synthetic peptides such as tripeptide-copper complex consist of glycyl-histidyl-lysine-Cu2+ in which both wound healing and in vitro hair growth were notable. In present study the hair growth stimulatory effect of peptide-antioxidant-mineral compounds were investigated and screened. Seven peptide complex derivatives (VitC-AHK, VitC-AHK-Cu2+, VitC-DAHK-Cu2+, VitC-KGHK-Cu2+, DAHK-Cu2+, AcDAHK-Cu2+, KGHK-Cu2+) were synthesized in which either tri- or tetrapetide linked to both side with antioxidant vitamin C and copper ion. Among them a copper ascorbyl phosphate succinyl tripeptide-34 (VitC-AHK-Cu2+; named as Alotide) showed favorable effect on dermal papilla cell proliferation with no cytotoxicity. This peptide showed remarkable hair re-growth pattern in C57BL/6 mouse than others following topical application. NMR structure analysis suggest that this peptide maintains stabilized molecular bond with copper ion. Thus hair growth promoting effect might be related to structure-activity of copper-tripeptide.