Infrared spectroscopic imaging has, in the last ten years, developed into a powerful biophysical technique for the study of biological samples. The technique has been used by us, and others, for the study of skin, brain, bone and other biological samples. The generation of spatially resolved infrared spectroscopic images provides, in essence, a histological image of the endogenous distribution of chemical components (proteins, lipids, DNA) within a tissue section without the use of stains or probe molecules. As the techniques is based on infrared spectroscopy, the spatially resolved spectra of a tissue section can also be probed to generate images corresponding to structural information inherent in the spectra. The current presentation will demonstrate the unique utility of infrared spectroscopic imaging to the biophysical investigation of hair. By sectioning hair, and collecting spatially resolved infrared spectra of these samples, it is possible to generate an almost infinite set of images corresponding to any relevant feature of hair chemistry, anatomy, and organization. The vast history of infrared spectoscopy studies of keratin provides a base from which spatially resolved images of intact hair sections can now be generated. This presentation will provide an overview of infrared spectroscopic imaging, describe its utility for the study of biological samples, then focus on our initial studies of hair with particular emphasis, and examples, demonstrating the ability to generate spatially resolved images of changes in hair chemistry, the penetration of exogenous molecules into hair, and changes in lipid and protein distribution as a result of external stresses to the hair.